THE FASTEST SEPARATIONS OF ENANTIOMERS IN HPLC

Author: Tamar Khatiashvili
Co-authors: Iza Matarashvili, Alessandro Volonterio, Bezhan Chankvetadze, Rusudan Kakava
Keywords: enantiomers, chiral sulphoxides, mobile phase
Annotation:

The separation of enantiomers of several chiral sulphoxides was studied on cellulose tris(4-chloro-3-methylphenylcarbamate) chiral columns in high-performance liquid chromatography with ACN and the mixture of n-Hexane and 2-propanol as mobile phases. In order to shorten analysis time one should increase the flow rate of mobile phase and decrease the retention factor and separation selectivity. These can be managed by careful selection of chiral analyte, chiral selector, optimizing a mobile phase and its composition and by optimizing a content of a chiral selector in adsorbent. In the present study 2-(benzylsulfinyl)-N,N-dimethyl benzamide, 2-(benzylsulfinyl)-N-methyl benzamide, and 2-(benzylsulfinyl)-benzamide were used as chiral analytes. As the mobile phase ACN and the mixture of n-hexane and 2-propanol were used. After optimizing the above mentioned parameters the shortest baseline enantioseparation with the analysis time of 5-6 seconds was obtained. By careful selection of a structure of chiral analyte and chiral selector, as well as a content of chiral selector in the packing material, morphology of silica and separation conditions a baseline separation of enantiomers in few seconds can be achieved.